Table of Contents
Which enzymes join nucleotides together by forming phosphodiester bonds?
Enzyme activity During the replication of DNA, there is a hole between the phosphates in the backbone left by DNA polymerase I. DNA ligase is able to form a phosphodiester bond between the nucleotides.
What enzyme forms phosphodiester bonds in DNA?
DNA polymerases
The formation of a phosphodiester bridge is catalyzed by DNA polymerases. DNA polymerases catalyze the formation of a phosphodiester bond efficiently only if the base on the incoming nucleoside triphosphate is complementary to the base on the template strand.
What enzyme joins the DNA strands after replication?
DNA ligase
In Summary: Major Enzymes
| Important Enzymes in DNA Replication | |
|---|---|
| Enzyme | Function |
| DNA polymerase | Synthesizes the new DNA strand; also proofreads and corrects some errors |
| DNA ligase | Re-joins the two DNA strands into a double helix and joins Okazaki fragments of the lagging strand |
How do phosphodiester bonds form in DNA?
Phosphodiester bonds are the backbone of the strands of nucleic acid present in the life existing on Earth. Phosphodiester bond is formed when exactly two hydroxyl groups in phosphoric acid react with a hydroxyl group on other molecules forming ester bonds.
Where are phosphodiester bonds found in DNA?
Phosphodiester bonds are central to all life on Earth,[fn 1] as they make up the backbone of the strands of nucleic acid. In DNA and RNA, the phosphodiester bond is the linkage between the 3′ carbon atom of one sugar molecule and the 5′ carbon atom of another, deoxyribose in DNA and ribose in RNA.
What are the 6 enzymes involved in DNA replication?
DNA replication requires other enzymes in addition to DNA polymerase, including DNA primase, DNA helicase, DNA ligase, and topoisomerase.
What are the 5 enzymes involved in DNA replication?
The enzymes involved in the replication of prokaryotic DNA are DNA polymerase I to III, helicase, ligase, primase, sliding clamp, topoisomerase, and single-strand binding proteins (SSBs).