Table of Contents
- 1 Can you tell whether enzymes are bigger or DNA is bigger in molecular size how do you know?
- 2 Why are enzymes smaller than DNA?
- 3 Can you think and answer how a reporter enzyme?
- 4 What is bigger cell or DNA?
- 5 What is the purpose of the restriction enzyme in gel?
- 6 What happens when DNA is placed on an electric field?
Can you tell whether enzymes are bigger or DNA is bigger in molecular size how do you know?
Answer: We know that enzyme is a protein, and protein is synthesized from a small strand of DNA. Hence, DNA is bigger than enzymes in terms of molecular size.
Why are enzymes smaller than DNA?
Enzymes are smaller in size than DNA molecules. This is because DNA contains genetic information for the development and functioning of all living organisms. It contains instructions for the synthesis of proteins and DNA molecules.
Why DNA molecule is crucial for the cell survival?
DNA contains the instructions needed for an organism to develop, survive and reproduce. To carry out these functions, DNA sequences must be converted into messages that can be used to produce proteins, which are the complex molecules that do most of the work in our bodies.
Which is bigger protein or DNA?
DNA contains the genetic information of all living organisms. Proteins are large molecules made up by 20 small molecules called amino acids. All living organisms have the same 20 amino acids, but they are arranged in different ways and this determines the different function for each protein.
Can you think and answer how a reporter enzyme?
Thus, reporter enzymes can be used to monitor the transformation of host cells by foreign DNA besides being a selectable marker. Note: Another protein molecule called green fluorescent protein as a reporter molecule. It is a selectable marker.
What is bigger cell or DNA?
Within a cell, a DNA double helix is approximately 10 nanometers (nm) wide, whereas the cellular organelle called a nucleus that encloses this DNA can be approximately 1000 times bigger (about 10 μm).
How are DNA molecules separated in agarose electrophoresis?
This is how agarose electrophoresis separates different DNA molecules according to their size. The gel is stained with ethidium bromide so you can visualize how these DNA molecules resolved into bands along the gel. Southern blotting may also be used as a visualization technique for agarose gels.
How are unknown DNA fragments measured in electrophoresis?
Unknown DNA samples are typically run on the same gel with a “ladder.” A ladder is a sample of DNA where the sizes of the bands are known. So after you run out your sample, you can compare the unknown fragments to the ladder fragments and determine the approximate size of the unknown DNA bands by how they match up to the known bands of the ladder.
What is the purpose of the restriction enzyme in gel?
Typically, a DNA molecule is digested with restriction enzymes, and the agarose gel electrophoresis is used as a diagnostic tool to visualize the fragments. An electric current is used to move the DNA molecules across an agarose gel, which is a polysaccharide matrix that functions as a sort of sieve.
What happens when DNA is placed on an electric field?
When DNA is placed on a field with an electric current, these negatively charged DNA molecules migrate toward the positive end of the field, which in this case is an agarose gel immersed in a buffer bath.