Table of Contents
How do scientists remove a donor cells nucleus?
We use a suction pipette (B) to hold the egg cell steady and a glass needle (C) to remove the cell’s nucleus. We have gently pushed the glass needle through the tough shell that surrounds the egg cell. Next, we inject the nucleus (at arrow) from a donor cell into the enucleated egg cell.
What does removing a nucleus do?
If the nucleus is removed from the cell then the cell will not be able to function properly, it will not be able to grow. All the metabolic functioning of the cell will stop. Without nucleus the cell will lose its control. It can not carry out cellular reproduction.
What do you now call a cell with its nucleus removed?
Nuclear transfer, the introduction of the nucleus from a cell into an enucleated egg cell (an egg cell that has had its own nucleus removed).
How is nuclear transfer done?
Nuclear transfer is a form of cloning. The steps involve removing the DNA from an oocyte (unfertilised egg), and injecting the nucleus which contains the DNA to be cloned. In rare instances, the newly constructed cell will divide normally, replicating the new DNA while remaining in a pluripotent state.
What did Gurdon’s frog experiment prove?
Gurdon transplanted nuclei that exhibited the mutation into normal oocytes and found that the resulting tadpoles exhibited the traits associated with the mutated nuclei, proving that the frogs developed using the donor nucleus, not the irradiated host DNA.
Why can’t the cell survive without the nucleus?
Nucleus is the brain of the cell and controls most of its functions. Thus without a nucleus, an animal cell or eukaryotic cell will die. Without a nucleus, the cell will not know what to do and there would be no cell division. Protein synthesis would either cease or incorrect proteins would be formed.
What was the major conclusion from the Gurdon experiment?
In 1962, John Gurdon removed the nucleus of a fertilized egg cell from a frog and replaced it with the nucleus of a cell taken from a tadpole’s intestine. This modified egg cell grew into a new frog, proving that the mature cell still contained the genetic information needed to form all types of cells.